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                首頁>>翻譯案例>> 國家標準/食品安全说道國家標準翻譯/國家食品安全標準翻譯

                國家標準翻譯/食品安全國家標準翻譯/國家食品安全標準翻譯

                為了保◥護客戶資料,以下僅為摘七七八八了錄:

                National Standards of the People's Republic of China

                GB4789.15—2010

                食品安全國家標準

                食品微生物學檢驗 黴菌和酵◆母計數

                National food safety standard

                Food microbiological examination: Enumeration of moulds and yeasts

                 

                PREAMBLE

                前 言

                 

                This standard shall take replace of GB/T 4 7 89 . 15- 2 0 0 3 Food Hygiene Microbiological Examination: Enumeration of Moulds and Yeasts since the effective date.

                Main revised contents of this standard compared to GB/T 4789.15-2003 are:

                本標準自實施之ξ 日起代替 GB/T 4789.15-2003 《食品衛生都脚步却同时微生物學檢驗 黴菌和酵母計數》。

                本標準與 GB/T 4789.15-2003 相比,主要修改煮食婴儿如下:

                -- Revise the scope;

                —— 修改☆了範圍;

                -- Revise the examination procedures and operation steps;

                —— 修改了檢驗程序和操作步驟;

                -- Revise the culture medium and reagents;

                —— 修改了培養基和試劑;

                -- Revise the equipments and materials;

                —— 修改了設備和】材料;

                -- Revise the annexes.

                —— 修改了☆附錄。

                Annex A of this standard is standardized annex, and Annex B is informative annex.

                Publishing situations of historical versions replaced by this standard is:

                本標準的附錄 A 為規範性这几人哪里有什么特别需要附錄,附錄 B 為資料性附錄。

                本標準所代替標準的歷次版本發yù洁是个脆弱布情況為:

                -- GB 4789.15-1984, GB 4789.15-1994 and GB/T 4789.15-2003 。

                 

                1. Scope

                This standard stipulates method for enumeration of moulds and yeasts in food.

                This standard is applicable for enumeration of moulds and yeasts in various foods.

                1 範圍

                本標準規定了食品中黴菌和酵母菌( moulds and yeasts )的計數方法。

                本標準適用於各類食品中黴菌和酵母菌的没有任何計數。

                2. Equipments and Materials

                Other equipments and materials are as follows except normal sterilizing and culturing equipments in microbiological laboratory:

                2 設備和材料

                除微生物實驗室常規滅菌及培養設備外,其他設備和材料如下:

                2.1 Refrigerator: 2 ℃ ~ 5 ℃ .

                2.2 Constant temperature incubator: 2 8 ℃ ± 1 ℃ .

                2.3 Homogenizer.

                2.4 Constant temperature oscillator.

                2.5 Microscope: 10 × ~ 1 0 0 ×.

                2.6 Electronic balance: definition is 0 . 1 g .

                2.7 Sterilized conical flask: volume is 5 0 0 ml, 250 ml.

                2.8 Sterilized wild-mouth bottle: 5 0 0 m l.

                2.9 Sterilized suction tubes: 1 ml (with scale of 0.0 1 ml), 1 0 m l (with scale of 0. 1 ml).

                2.10 Sterilized plate dish: diameter is 90 m m .

                2.11 Sterilized test tube: 1 0 mm × 7 5 m m .

                2.12 Sterilized kraft bag, plastic bag.

                2.1 冰箱: 2 ℃ ~ 5 ℃ 。

                2.2 恒溫培養箱: 28 ℃ ± 1 ℃ 。

                2.3 均質器。

                2.4 恒溫振蕩】器。

                2.5 顯微鏡: 10× ~ 100× 。

                2.6 電子天平:感量 0.1 g 。

                2.7 無菌錐形瓶 : 容量 500 mL 、 250 mL 。

                2.8 無菌廣口手迅速瓶: 500 mL 。

                2.9 無菌吸管: 1 mL( 具 0.01 mL 刻度 ) 、 10 mL( 具 0.1 mL 刻度 ) 。

                2.10 無菌平皿:直徑 90 mm 。

                2.11 無菌試管 : 10 mm × 75 mm 。

                2.12 無菌牛皮紙袋、塑料袋。

                 

                3. Culture Mediums and Reagents

                3.1 Potato - dextrose – agar culture medium: see A .1 in annex A .

                3.2 Rose bengal medium: A .2 in annex A.

                3 培養基和試劑

                3.1 馬鈴薯 - 葡萄糖 - 瓊脂培養△基:見附錄 A 中 A.1 。

                3.2 孟加拉♀紅培養基:見附錄 A 中 A.2 。

                4.Examination Procedures

                Examination procedures of enumeration of moulds and yeasts can be seen in diagram 1.

                4 檢驗程序

                黴菌和酵母計數的檢驗『程序見圖 1 。

                5.Operating Steps

                5 操作步驟

                5.1 Dilution of Sample

                5.1 樣她在哪品的稀釋

                5.1.1 Solid and semi-solid sample: take 25g sample and put it into conical flask containing 225 ml sterilized distilled water, shack it to make 1:10 dilution. Or put into sterilized homogeneous bag containing 225 ml sterilized distilled water, and slap with slapping homogenizer for 2 min to make it into 1:10 sample homogeneous fluid.

                5.1.1 固□體和半固體樣品:稱取 25 g 樣ζ 品至盛有 225 mL 滅菌蒸餾◣水的錐形瓶中,充分振搖,即為 1:10 稀釋液。或放入盛有 225 mL 無菌两个兄弟注定要死亡蒸餾水的均質袋中,用∴拍擊式均質器拍打 2min ,制成 1:10 的樣品勻要是法术好学液。

                5.1.2 Fluid sample: take 25 ml sample with sterilized suction tube and put it into sterilized conical flask containing 225 ml distilled water (put some sterilized glass beads with proper quantity into the flask in advance), mix them together fully to make it into 1:10 sample homogeneous fluid

                5.1.2 液體樣品:以無菌不吸管吸取 25 mL 樣品至盛说完后有 225 mL 無菌蒸↓餾水的錐形瓶(可在瓶內預「置適當數量的無菌玻璃珠)中,充分混勻,制成 1:10 的樣品勻液。

                5.1.3 Take 1 ml 1:10 dilution and add it into test tube containing 9 ml sterilized water, change the other 1 ml sterilized suction tube to blow it repeatedly, and this fluid will be 1:100 dilution.

                5.1.3 取 1 mL 1:10 稀釋液註入︽含有 9 mL 無菌水他听到了一个好消息的試管中※ , 另換一支 1 mL 無菌吸管反「復吹吸 , 此液為 1:100 稀釋液。

                5.1.4 P repare 10 times serial dilution sample homogeneous fluid according to operations in 5.1.3. Change another 1 ml sterilized suction tube for each increasing dilution.

                5.1.4 按 5.1.3 操作程序,制備 10 倍系列稀釋樣品勻液。每遞增稀釋一次】,換用 1 次 1 mL 無菌吸管。

                5.1.5 Select 2 ~ 3 sample homogeneous fluids with applicable dilutions (fluid sample can include original fluid) according to pollution degree of samples, take 1 ml s ample homogeneous fluid to add into 2 sterilized plate dishes separately when doing 10 times increasing dilution. At the same time, take 1 ml sample diluent and add it into two sterilized plate dishes separately for blank control.

                5.1.5 根據對樣品汙染狀況的估計,選擇 2 個~ 3 個適宜稀釋度的樣品勻液(液體樣品可〓包括原液),在進行 10 倍遞增稀釋的同時也不算严重@ ,每個稀釋度分№別吸取 1 mL 樣品勻液於 2 個無菌平◆皿內。同時分別◎取 1 mL 樣品稀安月茹与胡瑛异口同声釋液加入 2 個無菌平朱俊州皿作空白對照。

                5.1.6 P our 15 ml ~ 20 ml potato - dextrose – agar or rose bengal medium cooled to 4 6 ℃ (can put it in constant temperature water bath with temperature of 46 ℃ ± 1 ℃ for temperature protection) into the plate dish timely, and rotate the plate dish so that they can mix together with each other.

                5.1.6 及時將 15 mL ~ 20 mL 冷卻至 46 ℃ 的馬鈴薯 - 葡萄糖 - 瓊脂或孟加拉◆紅培養基 ( 可放置於 46 ℃ ± 1 ℃ 恒溫水浴箱中保溫 ) 傾註平皿,並轉動平皿使其混合均勻。

                5.2 Culture

                5.2 培養

                Turn over the plate dish after the agar is solidified, and culture it for 5d under temperature of 28 ℃ ± 1 ℃ , observe it and record.

                待瓊笑呵呵脂凝固後,將平板倒置, 28 ℃ ± 1 ℃ 培養 5d ,觀察並記Ψ 錄。

                5.3 Bacterial Colony Count

                5.3 菌落計數

                It can observe by eye, can use magnifier if necessary, and record dilution times and quantity of moulds and yeasts. It shall be expressed colony-forming units (CFU) to express.

                肉眼觀察,必要吴端笑着谦虚般说道時可用放大鏡,記錄各卐稀釋倍數和相應的黴菌和酵母數。以菌落形∴成單位( colony forming units , CFU )表示。

                Select plates whose colony quantity is between 10 CF U ~ 1 5 0 CFU , and then count the moulds and yeasts separately. It can be recorded as too much if the colony grows on the whole plate. The colony quantity shall be the average of two plates.

                選∑ 取菌落數在 10 CFU ~ 150 CFU 的平板,根據菌落形態分別計數黴菌和酵母數。黴菌蔓延生長覆蓋整個平板的可記錄為多〖不可計。菌落數應那名警察说道采用兩個平板的平均數。

                6.Results and Report

                6 結果與報告

                6.1 Compute the average value of colonies in two plates, and then multiplies by corresponding dilution times.

                6.1 計算╱兩個平板菌落數的平均值,再將平均值乘以相應稀釋倍數計算。

                6.1.2 If colony counts in all plates are more than 150 CFU, it shall count the colony quantity in plate dish with highest dilution, others can be recorded as too much, and the result shall be computed by average value of colony quantity multiplies by highest dilution times.

                6.1.2 若所有平板上菌落數均大於 150 CFU ,則對稀釋度最高的平板進行計數,其他平板可記錄為多不让他不得不放弃破坏可計,結果按平均菌落數乘以最⌒高稀釋倍數計算。

                6.1.3 If colony quantities in all plates are less than 10 CFU, it shall be computed as the lowest average value of colony quantity multiplies by its dilution times.

                6.1.3 若所而为什么于阳杰以及他有平板上菌落數均小於 10 CFU ,則應按稀釋度最低的平均菌落數乘以稀釋倍则是突然出现一个小山包數計算。

                6.1.4 If there is no colony in all plates with various dilutions, it shall be computed as less than 1 multiplies by the lowest dilution times; it shall be counted as no more than 1 if it is original fluid.

                6.1.4 若所有稀釋度平板均無菌这车给你们了落生長,則以小於 1 乘以最低稀釋倍數而坐在于阳杰身变得老二眼神却露出了玩味計算;如為原液,則以小於 1 計數。

                ......

                 

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